RT-PCR efficiency validation
Degraded RNA or impure RNA drastically reduces the yied of RT-PCR reactions, while RNA contamination in cDNA samples also reduces the labeling efficiency for micro-array experiments.
Furthermore, copurified components as guanidinium salts present in the extraction buffers can also inhibit the reaction and should be detected to avoid unsuccessful experiments.
The DropSense96 platform combined with cDrop software provides a unique tool for both specific RNA quantification and purity evaluation by determining the contaminations present in the sample. Moreover our technology is able to asses the level of degradation of RNA samples by quantifying specifically nucleotides RNA as a marker of degradation.
This technology is ideal to help with:
- Specific quantification and QC of starting RNA before RT-PCR
- Evaluation of the efficiency of RT-PCR
- QC of synthesized cDNA