DNA contamination in RNA samples is present in varying amounts whatever the extraction method used. This leads to an overestimation of the RNA A260 absorbance measurement but could also affect downstream experiments.
For instance, residual DNA in RNA samples can lead to false positive results in a QPCR experiment. That’s why a DNase treatment is strongly advised. To date, there was no convenient method to assess the efficiency of the DNase treatment.
Trinean offers now the DropSense96 platform combined with cDrop software which provides a unique tool for both specific RNA quantification and purity evaluation by determining the presence of contaminations like DNA.
This technology is ideal to help with:
- Specific quantification of RNA
- Specific quantification of residual DNA
- Checking the purity of RNA sample