Proteins

The measurement of protein concentration in aqueous samples is an important assay in biochemistry research and protein production facilities. Spectrophotometric protein quantification assays are commonly used methods to rapidly determine the concentration of a protein.

They utilize the direct UV absorbance of the protein at 280nm in combination with its extinction coefficient or an indirect dye-based absorbance methods like BCA, Lowry and Bradford assays.

The DropSense96 is a multi-functional droplet plate reader fit for native absorbance and colorimetric protein quantification methods.
When using native protein absorbance at 280 nm, the DropSense96 with its DropPlate-D, provides accurate analysis across a broad range of concentrations spanning four orders of magnitude going up to 110 OD (10mm path conversion).  The use of colorimetric reagents also provides a linear range for protein quantification spanning several orders of magnitude with significant greater sensitivity relative to the A280 intrinsic protein quantification. Furthermore, the full UV-VIS detection allows multi-label analysis of fluorescent labeled peptides for accurate labeling efficiency analysis.
Overall, the Trinean DropSense combined with the microfluidic DropPlates or classic 96well microtiter plates, solves the current bottleneck of quantifying protein samples in a medium to high throughput protein production workflow while consuming a minimal amount of sample. The microfluidic DropPlates ensure high quality measurements by avoiding evaporation, dilution errors or meniscus effects.

Application note:

1.03 Micro-volume protein qusing the DropSense96 droplet plate reader.pdf

AN 1.14 Droplet UV-VIS quantification of native proteins in biopharmaceutical processing.pdf