The polymerase chain reaction (PCR) is a fundamental tool in molecular biology and clinical testing. Traditionally PCR-products are analyzed on a gel, but this still remains an elaborate and time-consuming technique.
A rapid and convenient method to assess the yield of the PCR- reaction would be desirable, in particular for higher throughput users.
UV-Vis quantification is simple and fast, but to date lacks the specificity needed for PCR amplicon quantification in a complex background of mainly nucleotides, and to a lesser extent the primers and enzymes.
The cDrop PCR quantification tool is a valuable alternative for existing fluorescence and capillary electrophoresis methods. Quantification of a 96well PCR plate is done in 5 minutes with a minimal amount of handling steps.