RNA contamination in DNA samples is observed in varying amounts whatever the extraction method used. This leads to an overestimation of the DNA A260 absorbance measurement and could also affect downstream experiments.
Residual RNA in DNA samples can inhibit kinases and polymerases during the Next Generation Sequencing process. That’s why a RNase treatment is required. To date, there was no cost effective and convenient method to assess the efficiency of the RNase treatment.
Trinean offers now the DropSense96 platform combined with cDrop software which provides an unique tool for both specific DNA quantification and purity evaluation by determining the contaminations present in the sample as for instance RNA.
This technology is ideal to help with:
- Optimization of RNase treatment during extraction workflow
- Specific quantification of DNA
- Specific quantification of residual RNA
- Checking the purity of DNA sample